Bio013573 1..8

نویسندگان

  • Beverley J. Glass
  • Rebecca G. Hu
  • Anthony R. J. Phillips
  • David L. Becker
چکیده

Connexins have been proposed as a target for therapeutic treatment of a variety of conditions. The main approaches have been by antisense or small peptides specific against connexins. Some of these peptides enhance communication while others interfere with connexin binding partners or bind to the intracellular and extracellular loops of connexins. Here, we explored the mechanism of action of a connexin mimetic peptide by evaluating its effect on gap junction channels, connexin protein levels and hemichannel activity in fibroblast cells under normal conditions and following ischemia reperfusion injury which elevates Cx43 levels, increases hemichannel activity and causes cell death. Our results showed that the effects of the mimetic peptidewere concentration-dependent. High concentrations (100-300 μM) significantly reduced Cx43 protein levels and GJIC within 2 h, while these effects did not appear until 6 h when using lower concentrations (10-30 μM). Cell death can be reduced when hemichannel opening and GJIC were minimised.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Bio013573 1473..1480

Connexins have been proposed as a target for therapeutic treatment of a variety of conditions. The main approaches have been by antisense or small peptides specific against connexins. Some of these peptides enhance communication while others interfere with connexin binding partners or bind to the intracellular and extracellular loops of connexins. Here, we explored the mechanism of action of a ...

متن کامل

Supplement to Internally 4-connected projective-planar graphs

A5 = {{1, 2}, {1, 3}, {1, 4}, {1, 5}, {2, 3}, {2, 4}, {2, 5}, {3, 4}, {3, 5}, {4, 5}, {6, 7}, {6, 8}, {6, 9}, {6, 10}, {7, 8}, {7, 9}, {7, 10}, {8, 9}, {8, 10}, {9, 10}} C11 = {{1, 2}, {1, 3}, {1, 4}, {1, 5}, {2, 3}, {2, 4}, {2, 5}, {3, 4}, {3, 5}, {4, 5}, {6, 9}, {6, 10}, {6, 11}, {7, 9}, {7, 10}, {7, 11}, {8, 9}, {8, 10}, {8, 11}} E42 = {{1, 4}, {1, 5}, {1, 6}, {2, 4}, {2, 5}, {2, 6}, {3, 4},...

متن کامل

4.3 Modiied Variants and Weaknesses 4 the Attack on Lucifer 3 Conditional Characteristics

Round output diierence input diierence p icb key fraction P = (f7g; 0) 1 A = 0 a = 0 1 1 2 B = f45g b = f7g p = 1=8 0 1/2 3 C = f6g c = f45g p = 1=8 1 1/2 4 f45g f6; 7g p = 1=8 0 1/2 5 0 0 1 1 6 f45g f6; 7g p = 1=8 0 1/2 7 f6g f45g p = 1=8 1 1/2 8 f45g f7g p = 1=8 0 1/2 9 0 0 1 1 8 f39g f7g p = 1=8 1 1/2 9 f7g f39g p = 1=8 1 1/2 T = (0; f39g) 17 ent fractions of the key space. Round output diie...

متن کامل

(Mart\355nez Mendoza, S. \(2007\)...)

7 ! +, %.3, , 8 5 1 & , 93 3 1 : 1 , . , , . , ;; + 3 1 : / 93 . 1 . / ! < %.3, 93 , . , 8 . / . 3 , , . ;01, . ; , . / 5 / . ' , = 1 > : , . , 8. . > , 3 . /, , . , .. , & ' 1 8 . . , . ' , 1: > , 3 3 ,% 1 : 93 1 , . 3 . . / 8. . . % > , ., = , , 93 . . & , %.3, > 93 5 . 8, 3 , .. / 3. 93 , 93 . , . . / , . ;01, . !! < %.3, 93 . 3 / , . , . , . 01, . > 3 2, , . , 8. . 3 . / , . . .. . 3 . . / ...

متن کامل

Probabilistic Name and Address Cleaning and Standardisation

! " $#% '&"( ) *+ ,$ . / 10 " 23 4 / ,5 $ 4 6 *7 6 / 1 8 9 : ; ,8 #< = > ! /*? 8 2 = @ / 1 A B1 4 < = *@ 4 8 CD FEG 1 ,H&I )EG < *J ,8 8 C = 7EK D 8 5*@ 8,8 8 CJ,8 L ( > 8 G 9 / M*@ "&7,8 0 C H 8 ,N '2 *7 8 ,C/ 8 ,M ' G ,H ,L 1 ' A OP 9 ,H&"(Q *7 J R S > 9 R 2 6 / 10 L = = R RT/ &1 C; 9 *@ (K ,8,H&REG H U /*7 1 '0 C < + 23 ',-,8 8 CU V W&123 /C = 2 8 ,F 4 AX Y 8 ' ' Z 8*723 = " + 4 R = ) 8 + = ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2015